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Stream cytometry can be used to evaluate the efficiency of differentiation protocols Along with the STEMdiff™ system, by taking a look at specific marker expression at Just about every phase on the differentiation process. It can be useful for other applications, which includes cell sorting, immunophenotyping, and purity evaluation.
Get the job done speedily at this phase to pool wells into a fifteen mL 지방흡입 tube. Observe: For a big-scale dissociation, use a multichannel pipette to pool cells right into a sterile reagent 지방흡입 reservoir. Incorporate the pooled wells into a fifteen mL tube.
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Whilst Now we have circuitously analyzed nasal epithelial cells in-house, there are publications that report PneumaCult™ supporting this cell type.
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TEER measurements may be executed regularly, without having causing harm to the cell Stem cell tradition. You could carry out a weekly TEER time course to explain the barrier function through the entire process of ALI culture differentiation.
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Yes, you’ll find the phase-by-move protocol for TEER measurement to evaluate the epithelial barrier integrity in ALI cultures listed here.
This can be an stop-level protocol created to dissociate PSC-derived kidney organoids into an individual-cell suspension and fixation for downstream move cytometry Evaluation.
The following fluorochrome-conjugated antibodies and dyes are recommended to aid Assessment of gene-edited CD34+ HSPCs: